Characterization of the Adhesin of Escherichia coli F18 Fimbriae

Characterization of porcine-specific surface (S-) layer protein carrying Lactobacillus species, S-layer proteins and the adhesin of Escherichia coli F18 fimbriae – potential applications for veterinary medicine

6 ABBREVIATIONS 9 LIST OF ORIGINAL PUBLICATIONS 11

Nanobody Mediated Inhibition of Attachment of F18 Fimbriae Expressing Escherichia coli

Post-weaning diarrhea and edema disease caused by F18 fimbriated E. coli are important diseases in newly weaned piglets and lead to severe production losses in farming industry. Protective treatments against these infections have thus far limited efficacy. In this study we generated nanobodies directed against the lectin domain of the F18 fimbrial adhesin FedF and showed in an in vitro adherenc...

Chaperone-Usher Fimbriae of Escherichia coli

Chaperone-usher (CU) fimbriae are adhesive surface organelles common to many Gram-negative bacteria. Escherichia coli genomes contain a large variety of characterised and putative CU fimbrial operons, however, the classification and annotation of individual loci remains problematic. Here we describe a classification model based on usher phylogeny and genomic locus position to categorise the CU ...

Direct evidence that the FimH protein is the mannose-specific adhesin of Escherichia coli type 1 fimbriae.

Type 1 fimbriae of Escherichia coli are surface organelles which mediate binding to D-mannose-containing structures. By direct binding of FimH to D-mannose attached to a carrier protein, we demonstrated that this protein was uniquely responsible for the receptor specificity. Furthermore, we show by receptor immunoelectron microscopy that the FimH protein is located laterally in the structure of...

Identification and Purification of F11 Fimbriae on Avian Pathogenic Escherichia coli